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Probiotics Biofilm-Integrated Electrospun Nanofiber Membranes: A New Starter Culture for Fermented Milk Production
journal contribution
posted on 2019-03-06, 00:00 authored by Meng-Xin Hu, Ji-Nian Li, Qian Guo, Ya-Qian Zhu, Hong-Mei NiuElectrospun
nanofiber membranes are widely investigated in the
past few decades as candidates for tissue engineering, which can mimic
natural extracellular matrix (ECM) and improve cell adhesion, proliferation,
and expression on nanofiber membranes. However, the formation of bacterial
biofilms on nanofiber membranes and application of the biofilm-integrated
nanofiber membranes remain largely unknown. Here, electrospun cellulose
acetate nanofiber membranes are first utilized as scaffold materials
for Lactobacillus plantarum (L. plantarum) biofilm formation. Nanofiber membranes proved to be an excellent
scaffold for bacteria biofilm with high stability, where biofilms
were interlocked with nanofibers forming a cohesive structure. In
comparison with planktonic bacteria, L. plantarum biofilms on nanofiber membranes show excellent gastrointestinal
resistance. Instead of decreasing, the number of viable cells increased
after 3 h digestion in vitro. The L. plantarum biofilm-integrated
nanofiber membranes were used as reusable starter cultures for fermented
milk production showing excellent fermentative ability and higher
survival of L. plantarum during shelf life. The viable
cells in fermented milk remained at 11 log CFU/g throughout the reusable
batches, which is far above the required value of 7 log CFU/g in commercial
products. In addition, the produced fermented milk possesses shorter
fermentation time and higher survival of probiotics during shelf life.
The results suggest electrospun nanofiber membranes are ideal scaffold
materials for bacteria biofilms immobilization in biotechnology and
fermentation engineering, which broaden the potential use of electrospun
nanofiber membranes in microbiology and strengthen the application
of biofilms in fermentation engineering.
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Probiotics Biofilm-Integrated Electrospun Nanofiber Membranesshelf lifeNew Starter Culturebacteria biofilms immobilizationscaffold materialsnanofiber membranesECMFermented Milk Production Electrospun nanofiber membranesnanofiber membranes show3 h digestionelectrospun cellulose acetate nanofiber membraneselectrospun nanofiber membranesbiofilm-integrated nanofiber membranesplantarum biofilm-integrated nanofiber membranesCFU
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