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Preparation and Characterization of Liposome-Encapsulated Plasmid DNA for Gene Delivery
journal contribution
posted on 2013-07-23, 00:00 authored by Rachel
M. Levine, Timothy R. Pearce, Maroof Adil, Efrosini KokkoliThe success of common nonviral gene
delivery vehicles, lipoplexes
and polyplexes, is limited by the toxicity and instability of these
charged molecules. Stealth liposomes could provide a stable, safe
alternative to cationic DNA complexes for effective gene delivery.
DNA encapsulations in three stealth liposomal formulations prepared
by thin film, reverse phase evaporation, and asymmetric liposome formation
were compared, and the thin film method was found to produce the highest
yields of encapsulated DNA. A DNA quantification method appropriate
for DNA encapsulated within liposomes was also developed and verified
for accuracy. The effect of initial lipid and DNA concentrations on
the encapsulation yield and fraction of DNA-filled liposomes was evaluated.
Higher encapsulation yields were achieved by higher lipid contents,
while a higher fraction of DNA-filled liposomes was produced by either
lower lipid content or higher DNA concentration. Control of these
parameters allows for the design of gene delivery nanoparticles with
high DNA encapsulation yields or higher fraction of DNA-filled liposomes.
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gene deliveryphase evaporationcationic DNA complexesDNA encapsulationsstealth liposomeslipid contentDNA concentrationsDNA quantification methodnonviral gene delivery vehiclesliposome formationGene DeliveryThe successDNA encapsulation yieldsDNA encapsulatedfilm methodlipid contentsencapsulated DNADNA concentrationencapsulation yieldsstealth liposomal formulationsgene delivery nanoparticles
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