ao6b00144_si_001.pdf (1.56 MB)
Preparation and Binding Evaluation of Histamine-Imprinted Microspheres via Conventional Thermal and RAFT-Mediated Free-Radical Polymerization
journal contribution
posted on 2016-10-06, 08:13 authored by Edwin
F. Romano, Regina C. So, Scott W. Donne, Clovia I. HoldsworthElevated histamine
(HTM) levels are closely linked to food poisoning
as well as to pathophysiological allergic diseases. In this study,
HTM-imprinted, solution-processable microspheres were prepared via
high-dilution conventional thermal polymerization (CTP) and controlled
radical polymerization (CRP) using ethylene glycol dimethacrylate
(80 or 90 wt %) and methacrylic acid at 60 °C in acetonitrile
and evaluated as recognition materials for sensing applications. The
polymers were selective to HTM in binding studies, cross-rebinding,
and competitive binding assays against the HTM analogues histidine,
imidazole, and tryptamine. The selective binding capacity was significantly
higher with CTP-80 (on the basis of mass: 21.0 μmol/g and surface
area: 8.08 × 10–2 μmol/m2)
than that with both CTP-90 (8.47 μmol/g, 4.49 × 10–2 μmol/m2) and CRP-80 (9.00 μmol/g,
1.19 × 10–2 μmol/m2).