Postsynthetic Conjugation of Protected Oligonucleotides Containing 3‘-Alkylamines

1998-03-31T00:00:00Z (GMT) by Dustin L. McMinn Marc M. Greenberg
The expansion of a highly efficient, convergent method for synthesizing 3‘-oligonucleotide conjugates is described. 3‘-Oligonucleotide conjugates containing amide or urea linkages between the oligonucleotide portion and the conjugated species were obtained by reacting protected oligonucleotides containing 3‘-alkylamines in aprotic organic solvents with carboxylic acid and isocyanate substrates, respectively. The protected oligonucleotides are obtained via standard automated synthesis on a photolabile solid-phase synthesis support. Excellent yields (83−100%) of bioconjugates were obtained using carboxylic acids and aryl isocyanates with as few as 5 molar equivalents of conjugation reagents relative to protected oligonucleotide. More moderate yields were obtained using alkyl isocyanates as substrates (70−88%). In addition, this method has proven to be useful for synthesizing complementary oligonucleotide−peptide conjugates from a single oligonucleotide, in which the polarity of the peptide with respect to the oligonucleotide is determined by the bond-forming process employed.