American Chemical Society
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Polymerase Chain Reaction Based Scaffold Preparation for the Production of Thin, Branched DNA Origami Nanostructures of Arbitrary Sizes

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posted on 2009-12-09, 00:00 authored by Elisabeth Pound, Jeffrey R. Ashton, Héctor A. Becerril, Adam T. Woolley
Designs for DNA origami have previously been limited by the size of the available single-stranded genomes for scaffolds. Here we present a straightforward method for the production of scaffold strands having various lengths, using polymerase chain reaction amplification followed by strand separation via streptavidin-coated magnetic beads. We have applied this approach in assembling several distinct DNA nanostructures that have thin (∼10 nm) features and branching points, making them potentially useful templates for nanowires in complex electronic circuitry.

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