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Plasmonic Nanosensors for Simultaneous Quantification of Multiple Protein–Protein Binding Affinities
journal contribution
posted on 2014-10-08, 00:00 authored by Rubén Ahijado-Guzmán, Janak Prasad, Christina Rosman, Andreas Henkel, Lydia Tome, Dirk Schneider, Germán Rivas, Carsten SönnichsenMost
of current techniques used for the quantification of protein–protein
interactions require the analysis of one pair of binding partners
at a time. Herein we present a label-free, simple, fast, and cost-effective
route to characterize binding affinities between multiple macromolecular
partners simultaneously, using optical dark-field spectroscopy and
individual protein-functionalized gold nanorods as sensing elements.
Our NanoSPR method could easily become a simple and standard tool
in biological, biochemical, and medical laboratories.