Plasmonic Nanosensors for Simultaneous Quantification of Multiple Protein–Protein Binding Affinities
2014-10-08T00:00:00Z (GMT) by
Most of current techniques used for the quantification of protein–protein interactions require the analysis of one pair of binding partners at a time. Herein we present a label-free, simple, fast, and cost-effective route to characterize binding affinities between multiple macromolecular partners simultaneously, using optical dark-field spectroscopy and individual protein-functionalized gold nanorods as sensing elements. Our NanoSPR method could easily become a simple and standard tool in biological, biochemical, and medical laboratories.