Phytotransformation and Metabolic Pathways of ¹⁴C‑Carbamazepine in Carrot and Celery

Carbamazepine (CBZ) is an anticonvulsant pharmaceutical compound of environmental concern due to its persistence, bioactive toxicity, and teratogenic effects. Studies on the kinetics and metabolic pathways of CBZ in plant tissues are still limited. In the present study, the phytotransformation of ¹⁴C-CBZ was explored. The ¹⁴C detected in bound residues was lower than in extractable residues (>85% of the uptaken ¹⁴C radioactivity) in plant tissues. CBZ underwent appreciable transformation in plants. A large portion of accumulated ¹⁴C radioactivity (80.3 ± 6.4%) in the cells was distributed in the cell water-soluble fraction. A total of nine radioactive transformation products of CBZ were identified, three of which were generated in vivo due to the contraction of the heterocycle ring. The proposed metabolic pathways revealed that conjugation with glutathione or phenylacetic acid was the major transformation pathway of CBZ in plants, with the contribution of epoxidation, hydroxylation, methoxylation, methylation, amination, and sulfonation.