nl5b02859_si_002.avi (1.6 MB)
Peptide Bioink: Self-Assembling Nanofibrous Scaffolds for Three-Dimensional Organotypic Cultures
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posted on 2015-10-14, 00:00 authored by Yihua Loo, Anupama Lakshmanan, Ming Ni, Lai Ling Toh, Shi Wang, Charlotte A. E. HauserPrintable scaffolds with adequate
mechanical strength and stiffness are sought after to ensure viability
of printed cells and tissues. We report the first peptide bioinks–lysine-containing
hexapeptides that self-assemble into stable, nanofibrous three-dimensional
hydrogels with unprecedented stiffness of up to 40 kPa. These biocompatible
scaffolds support the three-dimensional culture of human stem cells
and differentiation of primary cells into organotypic (gastrointestinal
and skin) structures for high-throughput screening, diagnosis, and
tissue engineering.