sb4001728_si_002.zip (11.15 kB)
PR-PR: Cross-Platform Laboratory Automation System
dataset
posted on 2014-08-15, 00:00 authored by Gregory Linshiz, Nina Stawski, Garima Goyal, Changhao Bi, Sean Poust, Monica Sharma, Vivek Mutalik, Jay D. Keasling, Nathan J. HillsonTo
enable protocol standardization, sharing, and efficient implementation
across laboratory automation platforms, we have further developed
the PR-PR open-source high-level biology-friendly robot programming
language as a cross-platform laboratory automation system. Beyond
liquid-handling robotics, PR-PR now supports microfluidic and microscopy
platforms, as well as protocol translation into human languages, such
as English. While the same set of basic PR-PR commands and features
are available for each supported platform, the underlying optimization
and translation modules vary from platform to platform. Here, we describe
these further developments to PR-PR, and demonstrate the experimental
implementation and validation of PR-PR protocols for combinatorial
modified Golden Gate DNA assembly across liquid-handling robotic,
microfluidic, and manual platforms. To further test PR-PR cross-platform
performance, we then implement and assess PR-PR protocols for Kunkel
DNA mutagenesis and hierarchical Gibson DNA assembly for microfluidic
and manual platforms.