sb7b00248_si_007.avi (1.4 MB)
Optogenetic Control of Endoplasmic Reticulum–Mitochondria Tethering
media
posted on 2017-11-25, 00:00 authored by Fan Shi, Fuun Kawano, Seon-hye Emily Park, Shinji Komazaki, Yusuke Hirabayashi, Franck Polleux, Masayuki YazawaThe
organelle interface emerges as a dynamic platform for a variety
of biological responses. However, their study has been limited by
the lack of tools to manipulate their occurrence in live cells spatiotemporally.
Here, we report the development of a genetically encoded light-inducible
tethering (LIT) system allowing the induction of contacts between
endoplasmic reticulum (ER) and mitochondria, taking advantage of a
pair of light-dependent heterodimerization called an iLID system.
We demonstrate that the iLID-based LIT approach enables control of
ER–mitochondria tethering with high spatiotemporal precision
in various cell types including primary neurons, which will facilitate
the functional study of ER–mitochondrial contacts.