jm8b01544_si_001.pdf (1.29 MB)
Optimization of Novel 1‑Methyl‑1H‑Pyrazole-5-carboxamides Leads to High Potency Larval Development Inhibitors of the Barber’s Pole Worm
journal contribution
posted on 2018-11-07, 00:00 authored by Thuy G. Le, Abhijit Kundu, Atanu Ghoshal, Nghi H. Nguyen, Sarah Preston, Yaqing Jiao, Banfeng Ruan, Lian Xue, Fei Huang, Jennifer Keiser, Andreas Hofmann, Bill C. H. Chang, Jose Garcia-Bustos, Abdul Jabbar, Timothy N. C. Wells, Michael J. Palmer, Robin B. Gasser, Jonathan B. BaellA phenotypic screen
of a diverse library of small molecules for
inhibition of the development of larvae of the parasitic nematode Haemonchus contortus led to the identification of
a 1-methyl-1H-pyrazole-5-carboxamide derivative with
an IC50 of 0.29 μM. Medicinal chemistry optimization
targeted modifications on the left-hand side (LHS), middle section,
and right-hand side (RHS) of the scaffold in order to elucidate the
structure–activity relationship (SAR). Strong SAR allowed for
the iterative and directed assembly of a focus set of 64 analogues,
from which compound 60 was identified as the most potent
compound, inhibiting the development of the fourth larval (L4) stage
with an IC50 of 0.01 μM. In contrast, only 18% inhibition
of the mammary epithelial cell line MCF10A viability was observed,
even at concentrations as high as 50 μM.
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focusanaloguePyrazoleSARStrongmethylinhibitionLHScompoundRHSPole0.01 μ MMethylsectionHigh Potency Larval Development Inhibitorsidentification0.29 μ McontrastassemblyelucidateOptimizationiterativelarvae50 μ Mrelationshipnematode Haemonchus contortusphenotypic1-IC 50concentrationepithelial cell line MCF 10A viabilitymodificationMedicinal chemistry optimization-5-carboxamidemoleculepyrazolescaffold
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