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On-Cell MAS NMR: Physiological Clues from Living Cells
journal contribution
posted on 2012-10-24, 00:00 authored by Giorgia Zandomeneghi, Karin Ilg, Markus Aebi, Beat H. MeierWhile structural information on biomolecules is mainly
obtained
from purified in vitro samples, NMR can also be applied in the context
of entire cells or organisms. The present study describes maturation
processes in living Salmonella enterica serovar Typhimurium,
a prevalent cause for human gastroenteritis. In our physiological
study, we follow the composition of the O-antigen on the outer bacterial
membrane with high-resolution MAS NMR spectroscopy. We detect and
characterize an evolution of the O-antigen composition, in particular
of the O-acetylation state of the O-antigen, a factor that can play
an important role in vaccine development.