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Nucleotide-Dependent Bioautocatalytic Timer Reaction
journal contribution
posted on 2016-05-27, 00:00 authored by Ting-Ru Chen, Ching-Fong Hsu, Chih-Lin Chen, Henryk A. Witek, Pawel L. UrbanWe
describe a biochemical timer composed of three biocatalytic
reactions involving three types of adenylate nucleotides: adenosine
triphosphate (ATP), adenosine diphosphate (ADP), and adenosine monophosphate
(AMP). The timer is triggered by a small amount of ATP or ADP. An
abrupt increase in the ATP concentration (following numerous amplification
cycles) leads to a sudden increase of luminescence from the reaction
mixture. The time point when the luminescence appears is found to
be a function of the initial concentration of the triggering nucleotide
(5.0 × 10–8–1.0 × 10–6 M), even in the presence of a complex biological matrix. The mechanism
of the observed dependence of the time of luminescence increase on
the concentration has been confirmed with simple kinetic models. Due
to the biocompatibility of the proposed trienzymatic reaction scheme
(sensitivity to common nucleotides and occurrence in a neutral pH
aqueous environment), the scheme can be used in bioengineered systems
that require modulation of the response time (light emission) by concentration.
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Keywords
amplification cyclesATP concentrationreaction mixturetrienzymatic reaction schemeNucleotide-Dependent Bioautocatalytic Timer Reactionadenosine monophosphatelight emissionAMPbioengineered systemsADPluminescence increaseadenosine triphosphateresponse timebiocatalytic reactionstime pointadenylate nucleotides
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