ie9b04413_si_001.pdf (1.49 MB)
Novel Nopinone-Based Turn-on Fluorescent Probe for Hydrazine in Living Cells with High Selectivity
journal contribution
posted on 2019-12-15, 14:29 authored by Mingxin Li, Jinfa He, Zhonglong Wang, Qian Jiang, Haiyan Yang, Jie Song, Yiqin Yang, Xu Xu, Shifa WangA novel turn-on fluorescent chemosensor 4 was designed
to distinguish highly toxic hydrazine (N2H4)
that relied on a new reaction mechanism. The probe 4 was
synthesized from nopinone, which is one of the natural product derivatives.
As expected, the developed probe 4 has high selectivity
towards hydrazine over various metal ions, anions, amino acids, and
other amines. The fluorescence intensity has a positive correlation
with the N2H4 concentration from 0 to 900 μM
and the limitation of detection was calculated to be 1.03 μM.
The possible mechanism of probe 4 detecting hydrazine
was confirmed by high-performance liquid chromatography (HPLC), high-resolution
mass spectrometry (HRMS), and density functional theory (DFT) analysis.
Moreover, the cytotoxicity and cell imaging experiments proved that
probe 4 has the ability to distinguish hydrazine in living
cells.