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Nonuniform Sampling Acquisition of Two-Dimensional Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for Increased Mass Resolution of Tandem Mass Spectrometry Precursor Ions
journal contribution
posted on 2017-08-08, 00:00 authored by Fabrice Bray, Julien Bouclon, Lionel Chiron, Matthias Witt, Marc-André Delsuc, Christian RolandoObtaining
the full MS/MS map for fragments and precursors of complex
mixtures without hyphenation with chromatographic separation by a
data-independent acquisition is a challenge in mass spectrometry which
is solved by two-dimensional (2D) Fourier transform ion cyclotron
resonance mass spectrometry (FTICR MS). Until now 2D FTICR MS afforded
only a moderate resolution for precursor ion since this resolution
is limited by the number of evolution interval steps to which the
number of scans, the acquisition time, and the sample consumption
are proportional. An overnight acquisition is already required to
reach a quadrupole mass filter-like unit mass resolution. Here, we
report that 2D FTICR MS using nonuniform sampling (NUS) obtained by
randomly skipping points in the first dimension corresponding to the
precursor selection gives access, after data processing, to the same
structural information contained in a complex mixture. The resolution
increases roughly as the inverse of the NUS ratio, up to 26 times
at NUS 1/32, leading to an acquisition time reduced in the same ratio
compared to a classical acquisition at the same resolution. As an
example, the analysis of a natural oil is presented.
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ratioion cyclotron resonance mass spectrometrymixtureTwo-Dimensional Fourier Transform Ion Cyclotron Resonance Mass Spectrometryevolution interval stepsacquisition timeprecursorNUSquadrupole mass filter-like unit mass resolutionNonuniform Sampling AcquisitionTandem Mass Spectrometry Precursor Ions Obtaining2 D FTICR MS
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