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Nondestructive Total Excitation–Emission Fluorescence Microscopy Combined with Multi-Way Chemometric Analysis for Visually Indistinguishable Single Fiber Discrimination
journal contribution
posted on 2016-02-10, 00:00 authored by Arsenio Muñoz de la Peña, Nirvani Mujumdar, Emily
C. Heider, Hector C. Goicoechea, David Muñoz de la
Peña, Andres D. CampigliaThe potential of total excitation–emission
fluorescence
microscopy combined with multiway chemometric analysis was investigated
for the nondestructive forensic analysis of textile fibers. The four
pairs of visually indistinguishable fibers consisted of nylon 361
dyed with acid yellow 17 and acid yellow 23, acetate satin 105B dyed
with disperse blue 3 and disperse blue 14, polyester 777 dyed with
disperse red 1 and disperse red 19, and acrylic 864 dyed with basic
green 1 and basic green 4. Excitation emission matrices were recorded
with the aid of an inverted microscope and a commercial spectrofluorimeter.
The full information content of excitation–emission matrices
was processed with the aid of unsupervised parallel factor analysis
(PARAFAC), PARAFAC supervised by linear discriminant analysis (LDA),
and discriminant unfolded partial least-squares (DU-PLS). The ability
of the latter algorithm to classify the four pairs of fibers demonstrates
the advantage of using the multidimensionality of fluorescence data
formats for the nondestructive analysis of forensic fiber evidence.