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Ninety-Day Nephrotoxicity Evaluation of 3‑MCPD 1‑Monooleate and 1‑Monostearate Exposures in Male Sprague Dawley Rats Using Proteomic Analysis
journal contribution
posted on 2020-02-21, 20:40 authored by Puyu Yang, Jinyu Hu, Junchen Liu, Yaqiong Zhang, Boyan Gao, Thomas T. Y. Wang, Lianzhou Jiang, Michael Granvogl, Liangli Lucy YuFatty
acid esters of 3-monochloropropane 1,2-diol (3-MCPD esters)
are processing-induced food toxicants, with the kidney as their major
target organ. For the first time, this study treated Sprague Dawley
(SD) rats with 3-MCPD 1-monooleate at 10 and 100 mg/kg BW/day and
1-monostearate at 15 and 150 mg/kg BW/day for 90 days and examined
for their potential semi-long-term nephrotoxicity and the associated
molecular mechanisms. No bodyweight difference was observed between
groups during the study. Both 3-MCPD 1-monooleate and 1-monostearate
resulted in a dose-dependent increase of serum urea creatinine, uric
acid and urea nitrogen levels, and histological renal impairment.
The proteomic analysis of the kidney samples showed that the 3-MCPD
esters deregulated proteins involved in the pathways for ion transportation,
apoptosis, the metabolism of xenobiotics, and enzymes related to endogenous
biological metabolisms of carbohydrates, amino acids, nitrogen, lipids,
fatty acids, and the tricarboxylic acid (TCA) cycle, providing partial
explanation for the nephrotoxicity of 3-MCPD esters.