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New Insights into the Coordination Chemistry and Molecular Structure of Copper(II) Histidine Complexes in Aqueous Solutions
journal contribution
posted on 2006-03-06, 00:00 authored by J. Gerbrand Mesu, Tom Visser, Fouad Soulimani, Ernst E. van Faassen, Peter de Peinder, Andrew M. Beale, Bert M. WeckhuysenAqueous solutions of Cu2+/histidine (his) (1:2) have been analyzed in parallel with infrared, Raman, ultraviolet/visible/near-infrared, electron spin resonance, and X-ray absorption spectroscopy in the pH range from 0 to 10.
Comprehensive interpretation of the data has been used to extract complementary structural information in order
to determine the relative abundance of the different complexes. The formation of six different, partly coexisting
species is proposed. Structural proposals from literature have been unambiguously confirmed, refined, or, in several
cases, corrected. At highly acidic conditions, Cu2+ and his are present as free ions, but around pH = 2, coordination
starts via the deprotonated carboxylic acid group. This results in the intermediate species Cu2+[H3his+(Oc)] and
Cu2+[H3his+(Oc)]2. The coordination via Oc is attended with a drop in the pKa value of the other receptor groups
resulting in a concomitant conversion to the bidentates Cu2+[H2his0(Oc,Nam)] and Cu2+[H2his0(Oc,Nam)]2, with the
latter being dominant at pH = 3.5. Coordination of the imidazole ring begins around pH = 3 and leads to the
formation of the mixed ligand complexes Cu2+[H2his0(Oc,Nam)][Hhis-(Oc,Nam,Nim)] and Cu2+[Hhis-(Nam,Nim)][Hhis-(Oc,Nam,Nim)] around pH = 5. It is demonstrated that coordination of the imidazole ring occurs predominantly
via the Nπ atom. At pH > 7, the double-tridentate ligand complex Cu2+[Hhis-(Oc,Nam,Nim)]2 is the major species with
the N atoms in the equatorial plane and the O atoms in the axial position. This complex decomposes at pH > 10
into a copper oxide/hydroxide precipitate. The overall results provide a consistent picture of the mechanism that
drives the coordination and complex formation of the Cu2+/his system.