nl300514v_si_001.pdf (1.64 MB)
Nanoscale E-Cadherin Ligand Patterns Show Threshold Size for Cellular Adhesion and Adherence Junction Formation
journal contribution
posted on 2012-04-11, 00:00 authored by Stine
H. Kristensen, Gitte A. Pedersen, Lene N. Nejsum, Duncan S. SutherlandThe role of ligand spatial distribution on the formation
of cadherin mediated cell–cell contacts is studied utilizing
nanopatterns of E-cadherin ligands. Protein patches ranging in size
from 100 to 800 nm prepared by colloidal lithography critically influence
adhesion, spreading, and formation of adherence junctions in epithelial
cells. Cells at 100 nm patterns show poor adhesion, while larger pattern
sizes show good adhesion, significant spreading, and defined cortical
actin. We estimate a threshold of 0.03 μm2 for epithelial
cellular attachment via E-Cadherin.