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Naked-Eye and Near-Infrared Fluorescence Probe for Hydrazine and Its Applications in In Vitro and In Vivo Bioimaging
journal contribution
posted on 2015-09-01, 00:00 authored by Jianjian Zhang, Lulu Ning, Jiting Liu, Jianxi Wang, Bianfei Yu, Xiaoyan Liu, Xiaojun Yao, Ziping Zhang, Haixia ZhangHydrazine has been applied diffusely
in most of the chemical industry;
however, it is a hazardous environmental pollutant and highly toxic
to organisms. Selective, rapid, and sensitive detection of hydrazine
thus becomes absolutely necessary in both biological and environmental
sciences. Accordingly, fluorescence probes for hydrazine have been
paid great attention in recent years. Disclosed here is the near-infrared
(NIR) fluorescence probe with a turn-on fluorescent probe CyJ based on the structure–emission property relationships of
the NIR dyes containing an acetyl group as the recognizing moiety.
This new probe not only can be readily prepared, but also shows excellent
sensing properties. First and most important of all, CyJ is highly selective for N2H4 over various
anions, cations, and other amino compounds and has a low limit of
detection (LOD) of hydrazine (5.4 ppb as fluorescence sensor and 6.1
ppb as UV sensor). Besides, CyJ exhibited a dramatic
increase in fluorescence at λmax = 706 nm in the
presence of N2H4, and it offers a rapid, colorimetric
and vapor sensing detection process for N2H4 in both aqueous solution and diluted human serum. Furthermore, CyJ has good cell-membrane permeability and low cytotoxicity.
In addition, we have successfully applied the CyJ to
visualize N2H4 in live mouse and, for the first
time, in tissues such as the liver, lung, kidney, heart, and spleen.