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N‑Cadherin Mimetic Peptide Nanofiber System Induces Chondrogenic Differentiation of Mesenchymal Stem Cells
journal contribution
posted on 2019-08-21, 15:33 authored by Cagla Eren Cimenci, Gozde Uzunalli Kurtulus, Ozum S. Caliskan, Mustafa O. Guler, Ayse B. TekinayCadherins
are vital for cell-to-cell interactions during tissue
growth, migration, and differentiation processes. Both biophysical
and biochemical inputs are generated upon cell-to-cell adhesions,
which determine the fate of the mesenchymal stem cells (MSCs). The
effect of cadherin interactions on the MSC differentiation still remains
elusive. Here we combined the N-Cadherin mimetic peptide (HAV-PA)
with the self-assembling E-PA and the resultant N-cadherin mimetic
peptide nanofibers promoted chondrogenic differentiation of MSCs in
conjunction with chondrogenic factors as a synthetic extracellular
matrix system. Self-assembly of the precursor peptide amphiphile molecules
HAV-PA and E-PA enable the organization of HAV peptide residues in
close proximity to the cell interaction site, forming a supramolecular
N-cadherin-like system. These bioactive peptide nanofibers not only
promoted viability and enhanced adhesion of MSCs but also augmented
the expression of cartilage specific matrix components compared to
the nonbioactive control nanofibers. Overall, the N-cadherin mimetic
peptide nanofiber system facilitated MSC commitment into the chondrogenic
lineage presenting an alternative bioactive platform for stem-cell-based
cartilage regeneration.
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Keywords
differentiation processesMSC differentiationsupramolecular N-cadherin-like systemcell-to-cell interactionscell-to-cell adhesionscell interaction sitematrix componentsalternative bioactive platformchondrogenic lineagechondrogenic factorsMesenchymal Stem Cells Cadherinspeptide nanofibersself-assembling E-PAstem-cell-based cartilage regenerationcadherin interactionsbioactive peptide nanofibersprecursor peptide amphiphile molecules HAV-PAMSC commitmentchondrogenic differentiationtissue growthpeptide nanofiber systemHAV peptide residuesnonbioactive control nanofibersextracellular matrix system
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