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NMR Spectroscopy Identifies Metabolites Translocated from Powdery Mildew Resistant Rootstocks to Susceptible Watermelon Scions
journal contribution
posted on 2015-09-16, 00:00 authored by Iqbal Mahmud, Chandrasekar Kousik, Richard Hassell, Kamal Chowdhury, Arezue F. BoroujerdiPowdery
mildew (PM) disease causes significant loss in watermelon.
Due to the unavailability of a commercial watermelon variety that
is resistant to PM, grafting susceptible cultivars on wild resistant
rootstocks is being explored as a short-term management strategy to
combat this disease. Nuclear magnetic resonance-based metabolic profiles
of susceptible and resistant rootstocks of watermelon and their corresponding
susceptible scions (Mickey Lee) were compared to screen for potential
metabolites related to PM resistance using multivariate principal
component analysis. Significant score plot differences between the
susceptible and resistant groups were revealed through Mahalanobis
distance analysis. Significantly different spectral buckets and their
corresponding metabolites (including choline, fumarate, 5-hydroxyindole-3-acetate,
and melatonin) have been identified quantitatively using multivariate
loading plots and verified by volcano plot analyses. The data suggest
that these metabolites were translocated from the powdery mildew resistant
rootstocks to their corresponding powdery mildew susceptible scions
and can be related to PM disease resistance.
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rootstockMickey LeePowdery Mildew Resistant Rootstocksmultivariate loading plotsmetaboliteSusceptible Watermelon ScionsPowdery mildewMahalanobis distance analysisvolcano plot analysesPM resistancescioncomponent analysisNMR Spectroscopy Identifies Metabolites TranslocatedSignificant score plot differencesdisease causesPM disease resistancewatermelon varietymanagement strategy
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