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Multistep Growth Mechanism of Calcium Phosphate in the Earliest Stage of Morphology-Controlled Biomineralization
journal contribution
posted on 2011-06-07, 00:00 authored by Takayuki Nonoyama, Takatoshi Kinoshita, Masahiro Higuchi, Kenji Nagata, Masayoshi Tanaka, Kimiyasu Sato, Katsuya KatoWe studied the effect of surface-functional-group position on precipitate morphology in the earliest stage of calcium phosphate biomineralization and determined the detailed mechanism of precipitation starting from nucleation to precipitate growth. The biomineralization template was a β-sheet peptide scaffold prepared by adsorption with carboxyl groups arranged at strict 7 Å intervals. Phosphate was then introduced. Within 10 s, highly ordered embryos of calcium phosphate were formed and confined by a peptide nanofiber pattern. They repeatedly nucleated and dissolved, with the larger embryos absorbing the smaller ones in a clear demonstration of an Ostwald-ripening-like phenomenon, then aggregated in a line pattern, and finally formed highly ordered nanofibers of amorphous calcium phosphate. This multistep growth process constitutes the earliest stage of biomineralization.
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