sb9b00534_si_001.pdf (1.83 MB)
Multiplexed Gene Expression Tuning with Orthogonal Synthetic Gene Circuits
journal contribution
posted on 2020-03-23, 12:44 authored by Mariola Szenk, Terrence Yim, Gábor BalázsiUnderstanding
the individual and joint contribution of multiple
protein levels toward a phenotype requires precise and tunable multigene
expression control. Here we introduce a pair of mammalian synthetic
gene circuits that linearly and orthogonally control the expression
of two reporter genes in mammalian cells with low variability in response
to chemical inducers introduced into the growth medium. These gene
expression systems can be used to simultaneously probe the individual
and joint effects of two gene product concentrations on a cellular
phenotype in basic research or biomedical applications.