ab9b01332_si_002.mp4 (4.39 MB)
Multifunctional Magnetic–Fluorescent Nanoparticle: Fabrication, Bioimaging, and Potential Antibacterial Applications
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posted on 2019-11-19, 08:29 authored by Yan Fang, Cuili Xing, Shixia Zhan, Meng Zhao, Mingxue Li, Hongling Liu, Chunzhang WangMagnetic–fluorescent nanoparticles integrating
imaging and
therapeutic capabilities have unparalleled advantages in the biomedical
applications. Apart from the dual ability of unique biomolecular fluorescent
recognition and magnetic modes, the nanoparticle also endows combined
effective therapies with high physiological stability, long-term imaging,
rapid response time, and excellent circulation ability. Herein, we
developed a carboxyl-functionalized magnetic nanoparticle that was
further functionalized by polydopamine (PDA) and Schiff base ligand
(3-aminopyridine-2-carboxaldehyde N(4)-methylthiosemicarbazone,
HL) to form multilayered coating single nanoparticles (Fe3O4@PDA@HL). Our work showed that the aggregation-induced
emission (AIE) effect could be produced by embedding In3+ into the Fe3O4@PDA@HL nanostructure, which
offered a new opportunity for utilization as a fluorescent detection
and therapeutic platform. Cellular fluorescent imaging experiments
provided bacterial cell biodistribution, demonstrating their excellent
luminescent performance, magnetic aggregation, and separation capability.
We simultaneously confirmed that the synergistic antibacterial effect
was closely related to both Fe3O4@PDA@HL and
In3+, leading to the disruption of membrane integrity and
the leakage of intracellular components, thus inducing bacterial death.
This approach presented in our work could promote the development
of future bioimaging and clinical therapy applications.