am5b00093_si_001.pdf (4.57 MB)
Multi-Domain Short Peptide Molecules for in Situ Synthesis and Biofunctionalization of Gold Nanoparticles for Integrin-Targeted Cell Uptake
journal contribution
posted on 2015-05-27, 00:00 authored by Hilal Unal Gulsuner, Hakan Ceylan, Mustafa O. Guler, Ayse B. TekinayWe describe design and synthesis
model of multidomain (modular) peptides (MDPs), which direct a reaction
cascade coupling the synthesis and surface functionalization of gold
nanoparticles (AuNPs) in a single step. The synthesis is achieved
via simple mixing of the aqueous solutions of auric acid and MDPs
at room temperature without the addition of any surfactants or toxic
intermediate reagents. This method allows facile control over the
nanoparticle size between ∼2–15 nm, which opens a practical
window for biomedical applications. In contrast to the conventional
citrate-mediated methods, peptide-mediated synthesis and stabilization
provide increased colloidal stability to AuNPs. As a proof of this
concept, we demonstrate active targeting of human breast adenocarcinoma
cell line (MCF7) using the one-step-prepared engineered AuNPs. Overall,
we propose a single-step, chemically greener, biologically safer method
for the synthesis and surface functionalization of gold nanoparticles
in a size-controlled manner. The chemical versatility of the MDP design
broadens the applicability of this strategy, thereby emerging as a
successful alternative for the currently available nanoparticle preparation
technologies.