pr500739v_si_003.pdf (196.63 kB)
Molecular Reprogramming of Arabidopsis in Response to Perturbation of Jasmonate Signaling
journal contribution
posted on 2014-12-05, 00:00 authored by Huizhuan Yan, Mi-Jeong Yoo, Jin Koh, Lihong Liu, Yazhou Chen, Dogukan Acikgoz, Qiaomei Wang, Sixue ChenJasmonates
(JAs) are important phytohormones that regulate a wide
range of plant processes including growth, development, senescence,
and defense. Jasmonate ZIM-domain (JAZ) proteins are repressors in
JA signaling. In Arabidopsis thaliana, 12 JAZ encoding genes were identified, but only a few have
been studied in detail. In this study, we focused on characterizing
the molecular networks involving JAZ2 and JAZ7. To understand the phenotypes and elucidate the regulatory
functions of JAZ2 and JAZ7, shoot
and root tissues from wild type (WT), jaz2, and jaz7 were harvested for RNA sequencing and metabolomics.
Distinct changes of transcripts and metabolites in JA biosynthesis,
primary and specialized metabolism, and oxidative stress were observed
among the three genotypes. In particular, many defense or stress-associated
metabolites and specialized metabolites were increased in response
to methyl jasmonate (MeJA) treatment. Most importantly, these changes
were subjected to quantitative modulation by the JAZ proteins at both
transcriptional and metabolic levels, the degree of which may control
resource allocation between growth and defense. This study not only
reveals MeJA-induced molecular reprogramming but also demonstrates
the functions of JAZ proteins as key regulators in fine-tuning JA
signal transduction.