mp9b00483_si_001.pdf (544.6 kB)
Molecular Evaluation of Oral Immunogenicity of Hepatitis B Antigen Delivered by Hydrogel Microparticles
journal contribution
posted on 2019-08-22, 16:03 authored by Xiang
Yi Chen, Adeel Masood Butt, Mohd Cairul Iqbal Mohd AminThe development of oral vaccine formulation is crucial
to facilitate an effective mass immunization program for various vaccine-preventable
diseases. In this work, the efficacy of hepatitis B antigen delivered
by bacterial nanocellulose/poly(acrylic acid) composite hydrogel microparticles
(MPs) as oral vaccine carriers was assessed to induce both local and
systemic immunity. Optimal pH-responsive swelling, mucoadhesiveness,
protein drug loading, and drug permeability were characterized by
MPs formulated with minimal irradiation doses and acrylic acid concentration.
The composite hydrogel materials of bacterial nanocellulose and poly(acrylic
acid) showed significantly greater antigen release in simulated intestinal
fluid while ensuring the integrity of antigen. In in vivo study, mice
orally vaccinated with antigen-loaded hydrogel MPs showed enhanced
vaccine immunogenicity with significantly higher secretion of mucosal
immunoglobulin A, compared to intramuscular vaccinated control. The
splenocytes from the same group demonstrated lymphoproliferation and
significant increased secretion of interleukin-2 cytokines upon stimulation
with hepatitis B antigen. Expression of CD69 in CD4+ T
lymphocytes and CD19+ B lymphocytes in splenocytes from
mice orally vaccinated with antigen-loaded hydrogel MPs was comparable
to that of the intramuscular vaccinated control, indicating early
activation of lymphocytes elicited by our oral vaccine formulation
in just two doses. These results demonstrated the potential of antigen-loaded
hydrogel MPs as an oral vaccination method for hepatitis B.