es051602r_si_001.pdf (27.46 kB)
Modulation of the Cytotoxicity and Genotoxicity of the Drinking Water Disinfection Byproduct Iodoacetic Acid by Suppressors of Oxidative Stress
journal contribution
posted on 2006-03-15, 00:00 authored by Eduardo Cemeli, Elizabeth D. Wagner, Diana Anderson, Susan D. Richardson, Michael J. PlewaDrinking water disinfection byproducts (DBPs) are
generated by the chemical disinfection of water and may
pose a hazard to the public health. Previously we
demonstrated that iodoacetic acid was the most cytotoxic
and genotoxic DBP analyzed in a mammalian cell system.
Little is known of the mechanisms of its genotoxicity. The
involvement of oxidative stress in the toxicity of iodoacetic
acid was analyzed with the antioxidants catalase and
butylated hydroxyanisole (BHA). Iodoacetic acid toxicity
was quantitatively measured with and without antioxidants
in Salmonella typhimurium strain TA100 and with Chinese
hamster ovary (CHO) cells. The endpoints included
cytotoxicity in S. typhimurium or in CHO cells, mutagenicity
in S. typhimurium, and genotoxicity in CHO cells. Neither
catalase nor BHA reduced the level of iodoacetic acid induced
cytotoxicity in S. typhimurium. In CHO cells neither
antioxidant caused a significant reduction in iodoacetic
acid induced cytotoxicity. However, in S. typhimurium, BHA
or catalase reduced the mutagenicity of iodoacetic acid
by 33.5 and 26.8%, respectively. Likewise, BHA or catalase
reduced iodoacetic acid induced genomic DNA damage
by 86.5 and 42%, respectively. These results support the
hypothesis that oxidative stress is involved in the induction
of genotoxicity and mutagenicity by iodoacetic acid.