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Modular Polymers as a Platform for Cell Surface Engineering: Promoting Neural Differentiation and Enhancing the Immune Response
journal contribution
posted on 2019-12-13, 22:29 authored by Yan Gu, Bing Liu, Qi Liu, Yingjie Hang, Lei Wang, John L. Brash, Gaojian Chen, Hong ChenRegulating cell behavior and cell fate are of great significance
for basic biological research and cell therapy. Carbohydrates, as
the key biomacromolecules, play a crucial role in regulating cell
behavior. Herein, “modular” glycopolymers were synthesized
by reversible addition–fragmentation chain transfer polymerization.
These glycopolymers contain sugar units (glucose), anchoring units
(cholesterol), “guest” units (adamantane) for host–guest
interaction, and fluorescent labeling units (fluorescein). It was
demonstrated that these glycopolymers can insert into cell membranes
with high efficiency and their residence time on the membranes can
be regulated by controlling their cholesterol content. Furthermore,
the behavior of the engineered cells can be controlled by modifying
with different functional β-cyclodextrins (CD-X) via host–guest
interactions with the adamantane units. Host–guest interactions
with the modular polymers were demonstrated using CD-RBITC (X = a
rhodamine B isothiocyanate). The glycopolymers were modified with
CD-S (X = seven sulfonate groups) and CD-M (X = seven mannose groups)
and were then attached, respectively, to the surfaces of mouse embryonic
stem cells for the promotion of neural differentiation and to the
surfaces of cancer cells for the enhancement of the immune response.
The combination of multiple anchors and host–guest interactions
provides a widely applicable cell membrane modification platform for
a variety of applications.
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cell behaviorPromoting Neural DifferentiationImmune Response Regulating cell behaviorCD-RBITCadamantane unitsresidence timecell fatecancer cellsβ- cyclodextrinssugar unitssulfonate groupshostinteractioncell therapyrhodamine B isothiocyanateglycopolymercell membrane modification platformCell Surface Engineeringmannose groupscell membranesCD-XModular PolymersCD-Scholesterol contentCD-M
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