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Mini 12, Miniature Mass Spectrometer for Clinical and Other ApplicationsIntroduction and Characterization
journal contribution
posted on 2015-12-17, 01:03 authored by Linfan Li, Tsung-Chi Chen, Yue Ren, Paul I. Hendricks, R. Graham Cooks, Zheng OuyangA benchtop miniature mass spectrometer
system, Mini 12, with ambient
ionization source and tandem mass spectrometry capabilities has been
developed and characterized. This instrument was developed as a self-contained
system to produce quantitative results for unprocessed samples of
small volumes including nonvolatile analytes. The ion processing system,
vacuum system, and control system are detailed. An integrated sample
loading system facilitates automated operation. A user interface has
been developed to acquire and to interpret analytical results for
personnel who have limited mass spectrometry knowledge. Peak widths
of Δm/z 0.6 Th (full width
at half-maximum) and a mass range of up to m/z 900 are demonstrated with the rectilinear ion trap mass
analyzer. Multistage experiments up to MS5 are accomplished.
Consumable cartridges have been designed for use in ambient paper
spray ionization, and the recently developed extraction spray ionization
method has been employed to improve the quantitative performance.
Monitoring of trace-levels of chemicals in therapeutic drugs, as well
as in food safety and environmental protection operations is demonstrated.
Dual MS/MS scans are implemented to obtain the intensities of the
fragment ions from the analyte and its internal standard, and the
ratio is used in quantitative analysis of complex samples. Limits
of quantitation (LOQ) of 7.5 ng/mL, with relative standard deviations
below 10%, have been obtained for selected therapeutic drugs in whole
blood throughout their individual therapeutic ranges.
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Multistage experimentsvacuum systemuser interfaceion processing systemunprocessed samplesmass spectrometer systemion trap mass analyzersample loading systemMS 5mass spectrometry knowledgefood safetyCharacterizationA benchtopcontrol systemnonvolatile analytesmass rangeambient ionization sourcetandem mass spectrometry capabilitiesConsumable cartridgesextraction spray ionization methodpeak widthsLOQambient paper spray ionizationprotection operationsfragment ions
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