ac5017776_si_002.mpg (22.99 MB)
Microfluidic Assay for Continuous Bacteria Detection Using Antimicrobial Peptides and Isotachophoresis
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posted on 2015-12-17, 05:20 authored by Ortal Schwartz, Moran BercoviciWe present a novel microfluidic assay
for continuous and quantitative
detection of bacteria in water. We leverage isotachophoresis (ITP),
an electrophoretic focusing technique, to create a stationary high
concentration zone of fluorescently labeled antimicrobial peptides
(AMPs) in a microfluidic channel. The tested water sample flows continuously
through this high concentration AMPs reaction zone; any bacteria present
in the sample is simultaneously labeled by, and separated from, the
high concentration AMPs. The labeled bacteria continue into the downstream
pure-buffer zone where the fluorescence signal is monitored, providing
a direct quantitative measurement of the original bacterial concentration
in the sample. We present the principles of the technique, demonstrate
its applicability for quantitative detection of E. coli as well as its stability over a 1 h monitoring time, and provide
a simple model for predicting its performance at different operating
conditions. The method could be potentially expanded for use with
other types of probes and provide continuous analysis and monitoring
of water samples at the point of need.
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Keywords
techniqueconcentration AMPs reaction zoneContinuous Bacteria Detectiondetectionleverage isotachophoresiswater samplesbacteriaantimicrobial peptidesconcentration zonefluorescence signalmicrofluidic channelconcentration AMPsnovel microfluidic assaywater sample flowsMicrofluidic AssayAntimicrobial Peptides1 h monitoring timeITP
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