np8b00425_si_001.pdf (5.89 MB)
Methionine-Containing Rhabdopeptide/Xenortide-like Peptides from Heterologous Expression of the Biosynthetic Gene Cluster kj12ABC in Escherichia coli
journal contribution
posted on 2018-10-10, 11:18 authored by Lei Zhao, Xiaofeng Cai, Marcel Kaiser, Helge B. BodeSeven new methionine-containing rhabdopeptide/xenortide-like
peptides (1–7) were identified from Escherichia coli expressing the rhabdopeptide/xenortide-like
peptide biosynthetic gene cluster kj12ABC from Xenorhabdus KJ12.1. Their structures were elucidated by
detailed HPLC-HR-MS/MS analysis and confirmed by chemical synthesis.
Bioactivity tests of these first rhabdopeptide/xenortide-like
peptide derivatives (2–7) showing
methionine building blocks compared to the usually found derivatives
containing exclusively hydrophobic amino acids such as valine, leucine,
or phenylalanine revealed good activities of 2–7 against protozoan parasites and no cytotoxicity against
mammalian L6 cells.