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Metallic Transition Metal Dichalcogenide Nanosheets as an Effective and Biocompatible Transducer for Electrochemical Detection of Pesticide
journal contribution
posted on 2018-08-29, 00:00 authored by Fengnian Zhao, Yao Yao, Xunjia Li, Lingyi Lan, Chengmei Jiang, Jianfeng PingOwing to their large
specific surface, favorable electrical conductivity,
and excellent electrocatalytic capabilities, two-dimensional transition
metal dichalcogenides have received considerable attention in the
field of biosensors. On the basis of these properties, we developed
a portable and disposable enzyme-based biosensor for paraoxon detection
using a metallic MoS2 nanosheets modified screen-printed
electrode (SPE). The exfoliated ultrathin metallic MoS2 nanosheets can accelerate the electron transfer on electrode surface
and contribute to the immobilization of acetylcholinesterase (AChE)
via the cross-linking of glutaraldehyde. Electrodeposited gold nanoparticles
(AuNPs) on SPE were used to immobilize MoS2 nanosheets
through the interaction between Au atoms on AuNPs and S atoms on MoS2. Using acetylcholine as the substrate, AChE can catalyze
the formation of electroactive thiocholine and further generate the
redox current. In the presence of paraoxon, the activity of AChE can
be inhibited, making the related electrochemical signals weaken. Under
the optimized conditions, this electrochemical biosensor exhibited
a favorable linear relationship with the concentration of paraoxon
from 1.0 to 1000 μg L–1, with the detection
limit of 0.013 μg L–1. Furthermore, this developed
biosensor was successfully applied to detect paraoxon in pretreated
apple and pakchoi samples, which can provide a reliable method for
the rapid analysis of organophosphorus pesticides in agricultural
products.
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Keywords
MoS 2 nanosheetsBiocompatible Transducertransition metal dichalcogenidesdetection limitelectrochemical signalsSPEpakchoi samplesMoS 2Metallic Transition Metal Dichalcogenide NanosheetsS atomsAuNPelectron transferelectrode surfaceoptimized conditionsexfoliated ultrathinimmobilize MoS 2 nanosheetsElectrochemical Detectionorganophosphorus pesticideselectroactive thiocholineelectrocatalytic capabilitiesAChEenzyme-based biosensorelectrochemical biosensorpretreated appleparaoxon detectionscreen-printed electrodeElectrodeposited gold nanoparticles
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