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Metal-Labeled Aptamers as Novel Nanoprobes for Imaging Mass Cytometry Analysis
journal contribution
posted on 2020-03-31, 20:29 authored by Youyi Yu, Jingqi Dang, Xiao Liu, Liping Wang, Shanhe Li, Ting Zhang, Xianting DingImaging
mass cytometry (IMC) is an emerging imaging technology
that exploits the multiplexed analysis capabilities of the CyTOF mass
cytometer to make spatially resolved measurements for tissue sections.
In a comprehensive view of tissue composition and marker distribution,
recent developments of IMC require highly sensitive, multiplexed assays.
Approaching the sensitivity of the IMC technique, we designed a novel
type of biocompatible metal-labeled aptamer nanoprobe (MAP), named 167Er-A10-3.2. The small molecular probe was synthesized by
conjugating 167Er-polymeric pentetic acid (167Er-DTPA) with an RNA aptamer A10-3.2. For demonstration, 167Er-A10-3.2 was applied for observing protein spatial distribution
on prostatic epithelium cell of paraffin embedded Prostatic adenocarcinoma
(PaC) tissue sections by IMC technology. The 167Er-A10-3.2
capitalizes on the ability of the aptamer to specifically bind target
cancer cells as well as the small size of 167Er-A10-3.2
can accommodate multiple aptamer binding antigen labeled at high density.
The detection signal of 167Er-A10-3.2 probe was 3-fold
higher than that of PSMA antibody probe for a targeted cell under
lower temperature epitope retrieval (37 °C) of PaC tissue. Furthermore,
we successfully demonstrated the simultaneously staining ability of
aptamer probes in IMC analysis. The successful imaging acquisition
using aptamers probes in IMC technology may offer opportunity for
the diagnosis of malignancies in the future.
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biocompatible metal-labeled aptamer nanoprobeprostatic epithelium cellPSMA antibody probeIMC technologybind target cancer cells167 Er-polymeric pentetic acidRNAmultiplexed analysis capabilities167 Er-A 10-3.2 probeCyTOF mass cytometeraptamer binding antigenImaging Mass Cytometry Analysis Imaging mass cytometrytissue sections167 Er-A 10-3.2.167 Er-A 10-3.2
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