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Mechano-Optical Analysis of Single Cells with Transparent Microcapillary Resonators
journal contribution
posted on 2019-12-12, 17:34 authored by Alberto Martín-Pérez, Daniel Ramos, Eduardo Gil-Santos, Sergio García-López, Marina L. Yubero, Priscila M. Kosaka, Álvaro San Paulo, Javier Tamayo, Montserrat CallejaThe study of biophysical properties of single cells is
becoming
increasingly relevant in cell biology and pathology. The measurement
and tracking of magnitudes such as cell stiffness, morphology, and
mass or refractive index have brought otherwise inaccessible knowledge
about cell physiology, as well as innovative methods for high-throughput
label-free cell classification. In this work, we present hollow resonator
devices based on suspended glass microcapillaries for the simultaneous
measurement of single-cell buoyant mass and reflectivity with a throughput
of 300 cells/minute. In the experimental methodology presented here,
both magnitudes are extracted from the devices’ response to
a single probe, a focused laser beam that enables simultaneous readout
of changes in resonance frequency and reflected optical power of the
devices as cells flow within them. Through its application to MCF-7
human breast adenocarcinoma cells and MCF-10A nontumorigenic cells,
we demonstrate that this mechano-optical technique can successfully
discriminate pathological from healthy cells of the same tissue type.
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Single Cellsresonator devicescell physiologyMCF -7cell biologyMechano-Optical AnalysisMCF -10A nontumorigenic cellsTransparent Microcapillary Resonatorscells flowlaser beamglass microcapillariestissue typebreast adenocarcinoma cellsresonance frequencycell stiffnessmechano-optical techniquehigh-throughput label-free cell classification
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