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Matrix-Assisted Ionization on a Portable Mass Spectrometer: Analysis Directly from Biological and Synthetic Materials
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posted on 2016-03-03, 00:00 authored by Zachary J. Devereaux, Christian A. Reynolds, Joshua L. Fischer, Casey D. Foley, Jessica L. DeLeeuw, James Wager-Miller, Srinivas B. Narayan, Ken Mackie, Sarah TrimpinMatrix-assisted
ionization (MAI)-mass spectrometry (MS) eliminates the need for high
voltage, a heat source, lasers, and compressed gases in the ionization
process and uses minimal solvents in sample preparation, thus making
MAI ideal for field-portable mass spectrometers. The broad applicability
of MAI is demonstrated by simple, rapid, and robust positive and negative
detection mode analyses of low and high mass compounds including some
pesticides, dyes, drugs, lipids, and proteins (186 Da to 8.5 kDa)
from various materials including urine, biological tissue sections,
paper, and plant material on a low pumping capacity, single-quadrupole
mass spectrometer. Different sample introduction methods are applicable,
including the use of a pipet tip or glass melting point tube, allowing
integration of sample preparation with sample introduction for increased
analytical utility and ease of operation, even when sampling directly
from surfaces.