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Magnetic Field Guided Chemotaxis of iMushbots for Targeted Anticancer Therapeutics
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posted on 2017-03-22, 00:00 authored by Tamanna Bhuyan, Amit Kumar Singh, Deepanjalee Dutta, Aynur Unal, Siddhartha Sankar Ghosh, Dipankar BandyopadhyayWe
report controlled migrations of an intelligent and biocompatible
“iMushbot” composed of Agaricus bisporus, mushroom microcapsules coated with magnetite nanoparticles. The
otherwise randomly moving microbot could meticulously direct itself
toward and away from the acid- and alkali-rich regions with the help
of acid, acidic catalase, and alkali stimuli, emulating the chemotaxis
of microorganisms. Although the catalytic decomposition of peroxide-fuel
in alkali engendered the directed alkali taxis toward higher pH region,
decomposition of peroxide fuel by the acidic catalase activity led
to directed acid taxis toward the lower pH region. The presence of
magnetite nanoparticles not only helped in improving the “activity”
of the motor through the heterogeneous catalytic decomposition of
the peroxide fuel but also provided a remote magnetic control on the
chemotaxis. The mesoporous iMushbots having negative ζ-potential
could easily be loaded with the cationic anticancer drugs, which were
magnetically guided toward the cancerous cells to cause apoptosis.
The iMushbots exhibited higher degree of drug retaining capacity inside
alkaline pH and showed facile drug release preferentially in the lower
pH environments. The experiments show the potential of the iMushbots
in retaining and transporting drugs in alkaline medium such as human
blood and releasing them in acidic medium such as the cancerous tissues
for cell apoptosis.