nn9b03982_si_001.pdf (4.91 MB)
Lysozyme-Assisted Photothermal Eradication of Methicillin-Resistant Staphylococcus aureus Infection and Accelerated Tissue Repair with Natural Melanosome Nanostructures
journal contribution
posted on 2019-08-23, 11:07 authored by Jun Li, Xiangmei Liu, Ziao Zhou, Lei Tan, Xianbao Wang, Yufeng Zheng, Yong Han, Da-Fu Chen, Kelvin Wai Kwok Yeung, Zhenduo Cui, Xianjin Yang, Yanqin Liang, Zhaoyang Li, Shengli Zhu, Shuilin WuPatients
often face the challenge of antibiotic-resistant bacterial
infections and lengthy tissue reconstruction after surgery. Herein,
human hair-melanosome derivatives (HHMs), comprising keratins and
melanins, are developed using a simple “low-temperature alkali
heat” method for potentially personalized therapy. The mulberry-shaped
HHMs have an average width of ∼270 nm and an average length
of ∼700 nm, and the negatively charged HHMs can absorb positively
charged Lysozyme (Lyso) to form the HHMs-Lyso composites through electrostatic
interaction. These naturally derived biodegradable nanostructures
act as exogenous killers to eliminate methicillin-resistant Staphylococcus aureus (MRSA) infection with a high antibacterial
efficacy (97.19 ± 2.39%) by synergistic action of photothermy
and “Lyso-assisted anti-infection” in vivo. Additionally, HHMs also serve as endogenous regulators of collagen
alpha chain proteins through the “protein digestion and absorption”
signaling pathway to promote tissue reconstruction, which was confirmed
by quantitative proteomic analysis in vivo. Notably,
the 13 upregulated collagen alpha chain proteins in the extracellular
matrix (ECM) after HHMs treatment demonstrated that keratin from HHMs
in collagen-dependent regulatory processes serves as a notable contributor
to augmented wound closure. The current paradigm of natural material–tissue
interaction regulates the cell–ECM interaction by targeting
cell signaling pathways to accelerate tissue repair. This work may
provide insight into the protein-level pathways and the potential
mechanisms involved in tissue repair.