la063251f_si_001.pdf (7.8 MB)
Localization of Sphingomyelin in Cholesterol Domains by Imaging Mass Spectrometry
journal contribution
posted on 2007-05-08, 00:00 authored by Carolyn M. McQuaw, Leiliang Zheng, Andrew G. Ewing, Nicholas WinogradThe location of each lipid in a palmitoyloleoylphosphatidylcholine/18:0 sphingomyelin/cholesterol monolayer
system is laterally resolved using imaging time-of-flight secondary ion mass spectrometry (TOF-SIMS) without the
necessity of adding fluorescent labels. This system of coexisting immiscible liquid phases shows cholesterol domains
with sizes and shapes comparable to those in the fluorescence microscopy literature. The results show that SM localizes
with cholesterol and that palmitoyloleoylphosphatidylcholine is excluded. Moreover, the segregation is not complete,
and there is a small amount of both phospholipids distributed throughout.
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sphingomyelinphasefluorescence microscopy literatureresults showlabelphospholipidLocalizationCholesterol Domainslaterallycoexisting immisciblepalmitoyloleoylphosphatidylcholinelipidion mass spectrometryImaging Mass SpectrometryThe locationimagingnecessitysegregationSphingomyelincholesterol domainsSM localizesmonolayer
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