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Local Heat Treatment for Suppressing Gastroduodenal Stent-Induced Tissue Hyperplasia Using Nanofunctionalized Self-Expandable Metallic Stent in Rat Gastric Outlet Model
journal contribution
posted on 2020-03-19, 11:38 authored by Jung-Hoon Park, Min Tae Kim, Kun Yung Kim, Nader Bakheet, Tae-Hyung Kim, Jae Yong Jeon, Wooram Park, Jorge E. Lopera, Dong-Hyun Kim, Ho-Young SongDespite the promising
results from the placement of covered or
uncovered self-expandable metallic stent (SEMS) as a nonsurgical therapeutic
option for the malignant gastric outlet obstruction (GOO), the long
patency of the stent is still limited because of stent-induced tissue
hyperplasia. Here, a local heat treatment using a nanofunctionalized
SEMS is proposed for suppressing stent-induced tissue hyperplasia
during GOO treatment. Highly efficient photothermal gold nanoparticle
(GNP) transducer-coated SEMSs (GNP-SEMSs) were prepared for local
heat treatment in rat gastric outlet. The in vivo heating temperature
in rat gastric outlet model was evaluated and compared with in vitro
heating temperature. Three groups of our developed 45 rat gastric
outlet models were used: group A, noncoated SEMS only; group B, GNP-SEMS
plus local heating; and group C, GNP-SEMS only to investigate in vivo
efficacy of GNP-SEMS mediated local heating. Ten rats per group were
sacrificed for 4 weeks, and five rats per group were sacrificed immediately
after local heat treatment. The in vivo heating temperature was found
to be 10.8% lower than the in vitro heating temperatures. GNP-SEMSs
were successfully placed through a percutaneous approach into the
rat gastric outlet (n = 45). The therapeutic effects
of GNP-SEMS were assessed by histologic examination including hematoxylin-eosin,
Masson trichrome, immunohistochemistry (TUNEL and CD31), and immunofluorescence
(Ki67), and the results showed significant prevention of tissue hyperplasia
following stent placement without adjacent gastrointestinal tissue
damage. GNP-SEMS-mediated local heating could be an alternative therapeutic
option for the suppression of tissue hyperplasia following stent placement
in benign and malignant GOOs.
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Suppressing Gastroduodenal Stent-Induced Tissue Hyperplasiaheat treatmentTUNELoutletNanofunctionalized Self-Expandable Metallic StentRat Gastric Outlet ModelGNP-SEMStissue hyperplasiaphotothermal gold nanoparticlestent placementstent-induced tissue hyperplasiaGNPLocal Heat TreatmentSEMSGOOvivo heating temperature
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