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LncRNA GAS5 and miR-137 Polymorphisms and Expression are Associated with Multiple Sclerosis Risk: Mechanistic Insights and Potential Clinical Impact
journal contribution
posted on 2020-05-12, 19:16 authored by Mahmoud
A. Senousy, Olfat G. Shaker, Noha H. Sayed, Nevine Fathy, Mona A. KortamThe pathogenesis of multiple sclerosis
(MS) is influenced by the
interaction of genetic and epigenetic mechanisms. The long noncoding
RNA GAS5 acts as a competing endogenous RNA for microRNA-137 and is
involved in demyelination. We investigated the association of GAS5
and miR-137 expression and their polymorphisms with MS susceptibility.
One hundred and eight MS patients and 104 healthy controls were included.
Expression analysis and genotyping of GAS5-rs2067079 and miR-137-rs1625579
single nucleotide polymorphisms were performed by qPCR. Serum GAS5
was upregulated, while serum miR-137 was downregulated in MS compared
with the controls. Serum miR-137 was an excellent discriminator of
MS patients from the controls (AUC = 0.97) and a negative independent
predictor of MS in multivariate logistic analysis. Serum GAS5 expression
was positively correlated with the expanded disability status scale
scores in the relapsing–remitting MS patients. The rs2067079TT
minor homozygote genotype was associated with an increased MS risk,
while the rs1625579G minor allele was protective. rs1625579 showed
an age-specific effect, while the rs2067079 affected the MS risk in
gender- and age-specific manners. In MS patients, rs2067079TT was
associated with a higher serum GAS5 than other genotypes, while serum
miR-137 did not differ between rs1625579 genotypes. Our results suggest
serum GAS5 and miR-137 as MS biomarkers, with miR-137 as a negative
predictor of MS risk and GAS5 as a marker of MS severity. We propose
rs2067079 and rs1625579 as novel genetic markers of MS susceptibility,
and at least, rs2067079 possibly impacts the crosstalk between GAS5
and miR-137.
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MS riskGAS 5rs 1625579MS patientsSerum GAS 5 expressionmiR -137 Polymorphismsrs 1625579 genotypesserum GAS 5Serum miR -137noncoding RNA GAS 5 actsrs 2067079Potential Clinical Impactserum miR -137LncRNA GAS 5disability status scale scoresrs 2067079TTAUCMultiple Sclerosis RiskSerum GAS 5miR -137 expressionMS susceptibilitymiR -1371625579G
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