je9b00452_si_001.pdf (1.11 MB)
Liquid–Liquid Equilibrium and Extraction Performance of Aqueous Biphasic Systems Composed of Water, Cholinium Carboxylate Ionic Liquids and K2CO3
journal contribution
posted on 2019-10-17, 12:11 authored by Mafalda
R. Almeida, Diana C. V. Belchior, Pedro J. Carvalho, Mara G. FreireIonic-liquid-based aqueous biphasic
systems (IL-based ABS) have
been broadly investigated for the separation of high-value compounds.
Nevertheless, the large-scale application of IL-based ABS is still
hampered by the high cost and hazardous features of most ILs used.
Aiming at characterizing novel ABS composed of ILs with a more acceptable
environmental footprint and enhanced biocompatibility, in this work,
ABS formed by water, cholinium carboxylate ILs ([Ch][CnCO2], with n = 2 to 6),
and K2CO3 were investigated. The respective
ternary phase diagrams, including binodal curves, tie-lines, and critical
points, were determined at (298 ± 1) K and atmospheric pressure.
The capability to form ABS (or of the IL to be salted-out) increased
with the increase of the alkyl chain length of the IL anion up to
cholinium pentanoate; however, for longer anion alkyl chain lengths
the ILs self-aggregation led to a decrease of the ILs ability to form
ABS. Furthermore, the liquid–liquid equilibrium data experimentally
determined were modeled using the local composition activity model
NRTL (nonrandom two liquid). The extraction performance of these systems
was finally evaluated with four nitrogenous bases (thymine, adenine,
guanine, cytosine). In all studied systems nitrogenous bases preferentially
migrated to the IL-rich phase, with extraction efficiencies ranging
between 81% and 97% in a single-step. The determined novel phase diagrams
indicate the composition of the mixtures required to use IL-based
ABS as separation routes. The extraction performance evaluation of
these systems with nitrogenous bases provides an indication of their
possible application to isolate high-value compounds with biotechnological
interest.