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Lipid Pore-Filled Silica Thin-Film Membranes for Biomimetic Recovery of Dilute Carbohydrates
journal contribution
posted on 2017-11-13, 00:00 authored by Shanshan Zhou, Daniel M. Schlipf, Emma C. Guilfoil, Stephen E. Rankin, Barbara L. KnutsonSelectively
permeable biological membranes containing lipophilic
barriers inspire the design of biomimetic carrier-mediated membranes
for aqueous solute separation. The recovery of glucose, which can
reversibly bind to boronic acid (BA) carriers, is examined in lipid
pore-filled silica thin-film composite membranes with accessible mesopores.
The successful incorporation of lipids (1,2-dipalmitoyl-sn-glycero-3-phosphocholine, DPPC) and BA carriers (4-((N-Boc-amino)methyl)phenylboronic acid, BAMP-BA) in the pores of mesoporous
silica (∼10 nm pore diameter) through evaporation deposition
is verified by confocal microscopy and differential scanning calorimetry.
In the absence of BA carriers, lipids confined inside the pores of
silica thin films (∼200 nm thick) provide a factor of 14 increase
in diffusive transport resistance to glucose, relative to traditional
supported lipid bilayers formed by vesicle fusion on the porous surface.
The addition of lipid-immobilized BAMP-BA (59 mol % in DPPC) facilitates
the transport of glucose through the membrane; glucose flux increases
from 45 × 10–8 to 225 × 10–8 mol/m2/s in the presence of BAMP-BA. Furthermore, the
transport can be improved by environmental factors including pH gradient
(to control the binding and release of glucose) and temperature (to
adjust lipid bilayer fluidity). The successful development of biomimetic
nanocomposite membranes demonstrated here is an important step toward
the efficient dilute aqueous solute upgrading or separations, such
as the processing of carbohydrates from lignocellulose hydrolysates,
using engineered carrier/catalyst/support systems.
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Keywords
14 increaseDilute Carbohydrates Selectivelysolute separationfactormolevaporation depositionBA carrierslipophilic barrierspH gradientlipid bilayersreversibly binddiffusive transport resistancelipid bilayer fluiditylignocellulose hydrolysatesBAMP-BAscanning calorimetryBiomimetic Recoveryglycero -3-phosphocholine DPPCboronic acidLipid Pore-Filled Silica Thin-Film Membranesbiomimetic nanocomposite membranesvesicle fusionbiomimetic carrier-mediated membranesconfocal microscopynmglucose flux increaseslipid pore-filled silica thin-film
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