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Linear Chain Formation of Split-Aptamer Dimers on Surfaces Triggered by Adenosine
journal contribution
posted on 2017-10-16, 00:00 authored by Chenze Lu, Christine Saint-Pierre, Didier Gasparutto, Yoann Roupioz, Eric Peyrin, Arnaud BuhotThe
detection of small molecules impacts various fields; however,
their small size and low concentration are usually the cause of limitations
in their detection. Thus, the need for biosensors with appropriate
probes and signal amplification strategies is required. Aptamers are
appropriate probes selected specifically against small targets such
as adenosine. The possibility to split aptamers in parts led to original
amplification strategies based on sandwich assays. By combining the
self-assembling of oligonucleotide dimers with split-aptamer dangling
ends and a surface plasmon resonance imaging technique, we developed
an original amplification approach based on linear chain formation
in the presence of the adenosine target. In this article, on the basis
of sequence engineering, we analyzed its performance and the effect
of the probe grafting density on the length of the chains formed at
the surface of the biosensor.
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Keywords
amplification approachadenosine targetprobedetectionchain formationSplit-Aptamer Dimerssandwich assaysamplification strategiessequence engineeringstrategymolecules impactssignal amplification strategiessplit aptamerssurface plasmon resonance imaging techniqueSurfaces TriggeredbiosensorLinear Chain Formationoligonucleotide dimers
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