np4010536_si_001.pdf (2.86 MB)
Lignans from the Fruit of Schisandra glaucescens with Antioxidant and Neuroprotective Properties
journal contribution
posted on 2014-06-27, 00:00 authored by Heng-Yi Yu, Zu-Yu Chen, Bin Sun, Junjun Liu, Fan-Yu Meng, Ye Liu, Tian Tian, An Jin, Han-Li RuanTwo rare 7,8-seco-lignans (1, 2), three
new lignan glycosides (3, 4a, 4b), and 10 known lignans (5–14) were
isolated from the fruit of Schisandra glaucescens Diels. The absolute configurations of 1 and 2 were determined by comparing their experimental and calculated electronic
circular dichroism spectra. The molecular structures of the new compounds
(3, 4a, and 4b), including
their absolute configurations, were determined using various spectroscopic
methods and hydrolysis reactions. The antioxidant activities of the
isolated compounds were tested using 2,2-diphenyl-1-picrylhydrazyl
and ferric reducing antioxidant power assays. Compounds 4, 7, 8, 10, 11, and 12 exhibited antioxidant activities of varying
potential in both assays. Of these compounds, 7 showed
the strongest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging
capacity, with IC50 values of 15.7 (150 μM DPPH)
and 34.6 μM (300 μM DPPH), respectively, and 4, 12, and 7 displayed higher total antioxidant
activities than Trolox in the ferric reducing antioxidant power assay.
The neuroprotective effects of these compounds against Aβ25–35-induced cell death in SH-SY5Y cells were also
investigated. Compounds 1, 2, 6, 7, 8, 11, and 12 exhibited statistically significant neuroprotective effects against
Aβ25–35-induced SH-SY5Y cell death compared
with the group treated only with Aβ25–35.