ja5b00795_si_001.pdf (1.06 MB)
Light-Triggered, Self-Immolative Nucleic Acid-Drug Nanostructures
journal contribution
posted on 2015-05-20, 00:00 authored by Xuyu Tan, Ben B. Li, Xueguang Lu, Fei Jia, Clarissa Santori, Priyanka Menon, Hui Li, Bohan Zhang, Jean J. Zhao, Ke ZhangThe
simultaneous intracellular delivery of multiple types of payloads,
such as hydrophobic drugs and nucleic acids, typically requires complex
carrier systems. Herein, we demonstrate a self-deliverable form of
nucleic acid–drug nanostructure that is composed almost entirely
of payload molecules. Upon light activation, the nanostructure sheds
the nucleic acid shell, while the core, which consists of prodrug
molecules, disintegrates via an irreversible self-immolative process,
releasing free drug molecules and small molecule fragments. We demonstrate
that the nanostructures exhibit enhanced stability against DNase I
compared with free DNA, and that the model drug (camptothecin) released
exhibits similar efficacy as free, unmodified drugs toward cancer
cells.