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Isolation and Synthesis of a Bacterially Produced Inhibitor of Rosette Development in Choanoflagellates
journal contribution
posted on 2016-03-21, 00:00 authored by Alexandra M. Cantley, Arielle Woznica, Christine Beemelmanns, Nicole King, Jon ClardyThe choanoflagellate Salpingoeca rosetta is a microbial marine eukaryote
that can switch between unicellular and multicellular states. As one
of the closest living relatives of animals, this organism has become
a model for understanding how multicellularity evolved in the animal
lineage. Previously our laboratories isolated and synthesized a bacterially
produced sulfonolipid that induces S. rosetta to form multicellular “rosettes.” In this study, we
report the identification of a bacterially produced inhibitor of rosettes (IOR-1) as
well as the total synthesis of this molecule and all of its stereoisomers.
Our results confirm the previously noted specificity and potency of
rosette-modulating molecules, expand our understanding of the complex
chemical ecology between choanoflagellates and rosette-inducing bacteria,
and provide a synthetic probe template for conducting further mechanistic
studies on the emergence of multicellularity.
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chemical ecologyf r osettesbacteriallymulticellular statesstereoisomerunicellularspecificitylaboratorychoanoflagellate Salpingoeca rosettai nhibitorRosette DevelopmentChoanoflagellateIORrosettesidentificationBacterially Produced InhibitorSynthesimulticellularityIsolationbacteriamoleculeunderstandingprobe templatesulfonolipidemergencemarine eukaryotepotencyanimal lineagemodelsynthesisorganism
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