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Interactions between Rotavirus and Suwannee River Organic Matter: Aggregation, Deposition, and Adhesion Force Measurement
journal contribution
posted on 2012-08-21, 00:00 authored by Leonardo Gutierrez, Thanh H. NguyenInteractions between rotavirus and Suwannee River natural
organic
matter (NOM) were studied by time-resolved dynamic light scattering,
quartz crystal microbalance, and atomic force microscopy. In NOM-containing
NaCl solutions of up to 600 mM, rotavirus suspension remained stable
for over 4 h. Atomic force microscopy (AFM) measurement for interaction
force decay length at different ionic strengths showed that nonelectrostatic
repulsive forces were mainly responsible for eliminating aggregation
in NaCl solutions. Aggregation rates of rotavirus in solutions containing
20 mg C/L increased with divalent cation concentration until reaching
a critical coagulation concentration of 30 mM CaCl2 or
70 mM MgCl2. Deposition kinetics of rotavirus on NOM-coated
silica surface was studied using quartz crystal microbalance. Experimental
attachment efficiencies for rotavirus adsorption to NOM-coated surface
in MgCl2 solution were lower than in CaCl2 solution
at a given divalent cation concentration. Stronger adhesion force
was measured for virus–virus and virus–NOM interactions
in CaCl2 solution compared to those in MgCl2 or NaCl solutions at the same ionic strength. This study suggested
that divalent cation complexation with carboxylate groups in NOM and
on virus surface was an important mechanism in the deposition and
aggregation kinetics of rotavirus.
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CaCl 2 solutionNaCl solutionsdivalent cation complexationSuwannee River Organic MatterrotaviruAFM70 mM MgCl 2. Deposition kineticsNOMMgCl 2 solutiondivalent cation concentrationinteraction force decay lengthExperimental attachment efficienciesquartz crystal microbalanceAtomic force microscopysurfaceAdhesion Force MeasurementInteractions30 mM CaCl 2virus
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