bm9b01128_si_001.pdf (1.69 MB)
Injectable Self-Healing Hydrogels Based on Boronate Ester Formation between Hyaluronic Acid Partners Modified with Benzoxaborin Derivatives and Saccharides
journal contribution
posted on 2019-10-14, 19:44 authored by Tamiris Figueiredo, Jing Jing, Isabelle Jeacomine, Johan Olsson, Thibaud Gerfaud, Jean-Guy Boiteau, Claire Rome, Craig Harris, Rachel Auzély-VeltyWe demonstrate here,
for the first time, formation of injectable
dynamic covalent hydrogels at physiological pH using benzoxaborin-saccharide
complexation as a reversible cross-linking method. The gels were prepared
by simply mixing hyaluronic acid modified with an original boronic
acid derivative, 3,4-dihydro-2H-benzo[e][1,2]oxaborinin-2-ol (1,2-ABORIN), and HA functionalized with 1-amino-1-deoxy-d-fructose. Dynamic rheological experiments confirmed the gel-like
behavior (storage modulus (G′) > loss modulus
(G″) in the frequency window explored) for
the designed HA-1,2-ABORIN/HA-fructose network. Furthermore, this
hydrogel exhibited excellent self-healing and injectability behaviors
in aqueous conditions and was found to be responsive to pH. Additionally,
fibroblast cells encapsulated in the HA network showed high viability
(>80% after 7 days of cell culture), as monitored by Live/Dead
staining.
Taken together, this new class of boronate ester cross-linked hydrogel
provides promising future for diverse biomedical applications.
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fibroblast cells encapsulatedHA networkInjectable Self-Healing Hydrogelsbenzoxaborin-saccharide complexationinjectability behaviorsABORINBenzoxaborin DerivativesPartners Modifiedboronate ester cross-linked hydrogelloss modulusHA functionalized-1-deoxy Dynamic rheological experimentscovalent hydrogelspHBoronate Ester Formationboronic acidstorage modulusfrequency windowcross-linking method7 daysgel-like behavior
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